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P-DIE and P-SUR mice were sacrificed in pairs (n = 11/each group) whenever a P-DIE mouse was identified.
After screening more than 300 pups, one such Msh2−/−Msh6+/− mouse was identified.
One mutant generated from this screen, the Cloth-ears (Clth) mouse, was identified by its poor startle response to sound.
A mouse was identified as positive for MMTV if immunolabeling for at least one of the markers was detected in the tumor or nearby tissues.
For each microsomic mouse detected (36 males and 38 females), the corresponding littermate control mouse was identified (37 males and 35 females).
IL-8 (a cytokine important for the function of human immune system, which has no single homologue in mouse) was identified as a key regulatory autocrine factor produced downstream of RANKL during osteoclastogenesis.
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A total of 121 cancers (14 to 25 per mouse) were identified in seven mice.
Orthologous genes between human, rat, and mouse were identified from NCBI HomoloGene, build 65.
Indeed, an anti-fractalkine antibody response in the NOD mouse is identified here.
The genotypes for each mouse were identified following the PCR genotyping method as described previously [ 14].
Orthologs between human and mouse were identified using reciprocal blast with the threshold of e-50.
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CEO of Professional Science Editing for Scientists @ prosciediting.com