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First, the Actb-MBS mouse was generated by knocking in 24 repeats of the MS2 stem-loop sequence in the 3′ untranslated region of the β-actin gene.
The ovarian cancer MISRII-SV40 transgenic mouse was generated by Dr. Denise Connolly's laboratory [45].
A PK2-EGFP transgenic mouse was generated by the GENSAT project at Rockefeller University [41].
Recently, an EWSR1 gene-targeted mouse was generated which displayed a very high post-natal lethality [6].
A chimeric male founder mouse was generated and crossed to normal C57BL/6 mice to generate heterozygous mice containing one copy of the mutated EpCAM allele.
The Lrp4 knockout (KO) mouse was generated by replacing the first exon with a neomycin resistance cassette using techniques described previously [15].
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CD4Cre Prdmice/fl mice were generated by crossing Prdm1fl/fl mice with CD4Cre mice.
Knockout mice were generated in collaboration with the University of California, Davis, Mouse Biology Program (MBP).
Cdh5-EGFP transgenic mice were generated by pronuclear injection into fertilized mouse oocytes.
H3mm7 KO mice were generated with a genome editing technique as described previously with several modifications19.
FKRP P448L mutant mice were generated by the McColl-Lockwood Laboratory for Muscular Dystrophy Research32,33.
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