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Dorsal skin of mouse was fixed in 10% neutral buffered formalin, embedded in paraffin, and sectioned at 4 µm.
The resin was allowed to set and the entire mouse was fixed by immersion in 4% buffered formalin.
During microscopy the mouse was fixed with a stereotactic device and maintained at 37°C by use of an appropriately pre-warmed heating mat.
The knee of the Balb/c mouse was fixed beyond 90° and 1×106 cells resuspended in 100 µl of PBS was injected into the distal femur using a 25 gauge needle.
The anesthesia was maintained during the recording by adding small amounts of ketamine-xylazine mixture every 30 min. The head of each mouse was fixed on the stage of the microscope (Labophoto, Nikon, Tokyo, Japan) using tooth and ear bars.
The mouse was fixed on a custom stereotaxic apparatus under a surgical microscope (Leica).
Similar(42)
Tissue sections from the obstructed and the contralateral kidneys of each mouse were fixed in 10% formalin in PBS and embedded in paraffin.
In Figure 1 human and mouse are ' fixed' species and chimpanzee and rat are ' variable' species.
For immunohistochemical staining, lung tissues in C57BL/6 mouse were fixed for at least 24 h by Bouin soultion.
The skin tissues from each mouse were fixed in buffered 10% formalin for 18 h and then embedded in paraffin.
The fluorescence of FITC was not detected when mice was fixed with PFA fixative before the perfusion of FITC-containing saline.
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