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Each mouse was exposed to the GO suspension via a single tail vein injection.
Briefly, an anesthetized pregnant mouse was exposed to an overdose inhalation of 70 100% CO2 gas for euthanasia, and then the uterus was extracted into cold PBS.
After being habituated in a new cage, each mouse was exposed to two identical objects placed in two corners of the apparatus during a 15-min training session (Figure S1).
The next day, the same mouse was exposed to both the familiar and novel environments after the temporary partition was removed, but without the animal being removed from the box.
Ten days later, when the xenografts formed in both hind legs, the right hind leg of each mouse was exposed to x-ray (5 Gy) and the left one was used as the mock-irradiation control.
The results of the salivary gland scores from the salivary gland squashes from all experiments in which a mouse was exposed to a single mosquito are reported in Table 2. Recognizing the sensitivity limitations of this semi-quantitative method, there was no indication that the sporozoite load was different in mosquitoes that transmitted or did not transmit an infection.
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The lower dorsal skin of mice was exposed to laser for 2 min at 0.3 W or i.d.
When other normal mice were exposed to the danger-scented air, they froze in their tracks.
Back in the laboratory, mice were exposed to the dust bunny particles.
When subsequent generations of mice are exposed to blood or nervous tissues from silent carrier mice, they get the disease.
The mice were exposed to particulate doses that were comparable to human doses allowed by the Environmental Protection Agency.
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CEO of Professional Science Editing for Scientists @ prosciediting.com