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The NOD-SCID mouse was considered the most appropriate host and 1×107 cells were xenografted in subsequent experiments.
Mouse was considered a random effect, while the medial or lateral glomerular location and the cage environment were considered fixed effects.
To initiate the test session, the mouse was placed on the central platform, facing an open arm, and was observed for 5 min. The mouse was considered to be on the central platform whenever two paws were on it, and in one of the arms when all four paws were inside.
The mouse was considered curling when the mouse twisted its trunk (Berrocoso et al., 2013).
Similarly, a mouse was considered to have left an arm if all four paws were placed outside the arm.
Because one-half of the offspring were non-TG mice, the EF121 mosaic mouse was considered to be a hemizygote.
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An 18-month-old mouse is considered to be equivalent in age to a 70-year-old person.
Both the dusky hopping mouse and the plains mouse are considered vulnerable by the International Union for the Conservation of Nature (IUCN), and the mulgara is listed as endangered in South Australia.
In this scheme, each mouse is considered to be a naive subject, and each performance is considered to be an independent observation [50].
The rd1 mouse is considered a relevant model for human RP, since about 4 5% of patients are suffering from mutations in the PDE6 beta gene [4].
The transcription factor Foxp3, known to inhibit several cytokine genes both in human and mouse, is considered as a good general marker of Tregs [40], [41].
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