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Noninvasive PAM of spleen in mouse was conducted similarily to the PAM experiment of liver.
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The ChIP-Seq experiments on mouse were conducted following the human ENCODE guidelines [ 30].
We recommend that combined studies of the distribution of ticks and white-footed mouse are conducted to assist decision-making on managing Lyme disease risk in Québec.
60 180 mg/kg daily gavage for mice was conducted for 7 weeks consecutively, and the result showed no clear influence on the hemogram, liver and spleen function.
Analysis of the distribution of the inflammatory phenotypes in the F2 mice was conducted using statistical modeling (Supplemental Figure S4).
The experimental protocol with the mice was conducted according to the European guidelines and was approved by the National Committee of the Scientific Research CNRSScientific Research CNRS
Evaluation of labeling for c-Fos between neglectful and nurturing MaD1 mice was conducted using a one-way ANOVA for each brain region.
Immunization of C57BL6 mice or Prnp−/− mice was conducted by inoculation of a mixture of equal doses of each virus (AdTRMet /AdCMNtTA and referred as AdhPrP) by the intramuscular route.
X-ray examination of the joints of arthropathic mice was conducted using a µFX-1000 µFX-1000microradigitalhy systemicroradiographyyo, Japan) system 25 kV and 80 µA with a 6-sec exposure with an imaging plate.
Genotyping of the mutant mice was conducted by PCR using the following primers: 5'-GAACAGAACAACAATAGCTTATGTTC-3' and 5'-CTTGTGTCATGCACAAAGTAGATGTCC-3' for the mutant allele, and 5'-GAACAGAACAACAATAGCTTATGTTC-3' and 5'-TAAGTGGATATTAGCCCAGAAACTTAG-3' for the WT allele.
Follow-up of individual mice was conducted.
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