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20 μL of blood (treated with Heparin sodium) from each mouse was collected for flow cytometry test respectively at Day 7, 14 or 21 after treatment, the percentage ratios of human CD3+ T cells to mouse lymphocytes in peripheral blood were showed.
Approximately 5 ml of BAL fluid per mouse was collected and pooled in sterile centrifuge tubes.
At day 6 p.i., and prior to the first deaths of untreated PbA-infected mice, 5 untreated PbA-infected mice (Group 1) and 5 oAC-treated PbA-infected mice (Group 2) were killed and fresh blood (300 500 µl per mouse) was collected and processed into RNA using a Mouse RiboPure™-Blood RNA isolation Kit (Ambion), according to the manufacturer's instructions.
‡1 positive A. pallipes mouse was collected from Sary-Chelek.
The serum of the mouse was collected and frozen at −80°C until assay.
The parasitized blood of each donor mouse was collected from the tail vein and diluted with 0.9% sodium chloride.
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The sera (300 μL for each mouse) were collected under anesthesia by extracting the eyeball blood.
Part of the spleen, part of the liver, and the complete right kidney of one mouse were collected and stored at −80 °C for ex vivo autoradiography evaluation.
Eyes from mouse were collected and placed in 4% paraformaldehyde.
At 3 dpi, the lungs of each mouse were collected, viral RNA extracted and sequenced.
Then, feces for each mouse were collected, counted and weighted at 0 8 h period.
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