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The tail blood of each mouse was checked for parasitemia up to 60 days postinfection; mice were culled when parasitemia was >10 trypanosomes/ml of blood.
At 10 weeks of age, single male and female mice were allowed to cohabit; every female mouse was checked each morning for the presence of a vaginal plug.
At the same time, as the NOD sequence was produced, the fidelity of the corresponding sequence in the C57BL/6J mouse was checked and assembly errors corrected where possible in conjunction with the GRC.
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The health status of infected mice was checked daily.
As for the adverse effects of injection of cahtepsin L inhibitor, when body weight, health condition, and a histopahtological examination of all organs from CatL-inh-administerred mice was checked, there was no significant change.
The genotype of the mice was checked using the following primers and PCR conditions: lacZ rose gene (forward: 5'-ATCCTCTGCATGGTCAGGTC-3', reverse: 5'-CGTGGCCTGATTCATTCC-3', 315 bp), endogenous gene (forward: 5'-CAAATGTTGCTTGTCTGGTG-3'; reverse: 5'-GTCAGTCGAGTGCACAGTTT-3', 210 bp).
The dissection of an equal number of bilateral areas from both wild type and knockout mice was checked by examination of the adhesive lid at the end of every laser capture microdissection session.
The viability status of the mice was checked daily.
The health status of tumor‐bearing mice was checked on a daily routine.
The health status of the mice was checked clinically on a daily basis and all mice were weighed on a weekly basis.
The survival of mice was checked daily, and the percent survival of the two groups were analysed by Kaplan Meier Plot.
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