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Specifically, the relevance of chemosignals released by an Ehrlich tumor-bearing mouse was assessed on the following: behavior (open-field and plus maze); hypothalamic NA levels and turnover; adrenaline (A) and NA plasmatic levels; and host resistance induced by tumor growth.
The number of tumours in each mouse was assessed by GFP fluorescence (as described previously [13]) at necropsy followed by histology.
Using an 8-point scale, the clinical score for each mouse was assessed starting at age 81 d until age 95 d.
A drifting grating of a pre-selected spatial frequency at 100% contrast appeared, and the mouse was assessed for tracking behavior for a few seconds.
Western blot analysis was performed as previously described.[10] The expression of TMEM135 in the mouse was assessed with peptide-specific rabbit polyclonal antibodies against TMEM135 (1∶1000) (generated in immunized rabbits by Invitrogen antibody services, Carlsbad, CA).
Of note, the visual ability of each mouse was assessed by suspending the animal by the tail and slowly lowering it toward a sold dark surface (a table) for three successive trials.
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In the subsequent 90 seconds, the mouse is assessed for reflexes, limb strength, and self-preservation actions.
Both eyes in each mouse were assessed.
About 30 cells per mouse were assessed in this study.
Ad35 biodistribution in mice was assessed following intravenous administration and compared with that of Ad5.
The effect of lead (Pb) ingestion on hematological parameters in male and female Swiss mice was assessed.
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