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When the mouse was added as an input device and when we learned how to use finger-based touch gestures for smartphones, we had to rethink how most of our core applications functioned.
L-NAME (0.2 g/kg mouse), was added to drinking water.
The second antibody (goat anti rabbit or donkey anti mouse) was added in 10% milk in PTW for 1 hr.
On the test day, to test the vocalization of male mice, one ovariectomized female mouse was first placed in a clean standard cage with regular bedding materials, and then a male mouse was added to pair with the female.
A1∶50 or 1∶10 dilution of wP-induced/naive or aP/adjuvant-induced serum samples from an individual mouse was added to the first well of each row and serially diluted 1∶2 across the plates.
On the test day, to test the vocalization of male mice, one female mouse chosen randomly was first put into a standard clean cage with new bedding materials, and then a male mouse was added to pair with the female.
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On Day 19 skinned minced mouse is added, and on Day 24 the fuzzy mice are eliminated.
Splenocytes from an uninfected mouse were added to each well at 5×105 cells/well in 50 µL complete medium to serve as antigen-presenting cells.
The volumes of all tumors from each mouse were added to give the overall tumor burden per animal.
In brief, wP- or wH-induced/naive serum samples (1 200 dilution) or aP/adjuvant-induced serum samples (1 50 dilution) from each individual mouse were added to and serially diluted 1 2 across plates coated with heat-inactivated exponential-phase bacteria.
A standard serum composed of a mixture of sera from the arthritic mice was added to each plate in serial dilutions and a standard curve was constructed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com