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mouse tumor specimens were fixed in 4% neutral-buffered formalin and embedded in paraffin.
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Furthermore, miR-21 and SOX2 showed up-regulation and overlapping expression pattern in RCAS/tv-a generated mouse brain tumor specimens.
Human tissue specimens were stained by Pax2 antibody; nude mice transplanted tumor specimens were stained by hematoxylin and eosin and PCNA antibody.
The same significant difference was observed between normal mouse lung and tumor specimens from lung tissues of mice carrying oncogenic K-ras [ 23].
In mice with evident tumor, specimens of the tumor tissue were also excised, snap frozen in liquid N2 and stored at −80°C.
In summary, using biochemistry, mouse genetics, and clinical tumor specimens we have found that SIRT1, a diet-responsive gene, is a regulator of β-catenin and has a tumor suppressive function.
(I, J ) IHC for CK19 on tumor specimens from mice of indicated genotypes.
Tumor specimens from mice were fixed in 10% formaldehyde, embedded in paraffin and sectioned into 5 μm thick slices.
Immunostaining analysis of cells and tumor specimens from mice or clinical patients was performed as described by Todaro et al. Details of immunohistochemistry and immunofluorencent assay are provided in the Supplementary Methods.
We found that DMC treatment more markedly increased the protein levels of poly-ubiquitinated proteins and CHOP compared with curcumin treatment in tumor specimens from nude mice harboring MDA-MB 435S cell xenografts.
Similar to the in vitro results, the expression levels of PTEN is upregulated, whereas Akt, p-Akt, FAK and XIAP in tumor specimens from hUCBSC-treated mice were prominently downregulated (Fig. 5A).
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