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MeWo human melanoma cells at a density of 2.5 × 10 cells in 0.1 mL serum-free medium were inoculated subcutaneously into the dorsal area of NSG mice to create the xenograft model.
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Contribution: K.L.T., T.S., J.M.S., P.A.J., S.J., N.E.B., and J.M.G. participated in designing and performing the research; R.S.-A., J.F., and S.D. contributed transgenic mice used to create the conditional KO system; and K.L.T. and J.M.G. wrote the paper.
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Certain activities, such as exploring a new environment, cause these mice to create receptors for the drug; and when they're given the drug later, the same neurons fire as did when the mice explored the new environment.
Twenty-two fvb mice were used to create the I-Long SAGE hypothalibraryibrary (Sanger_Hypo).
In the first experiment, we transplanted BM3.3 bone marrow into non-lethally irradiated CBA mice to create synchimeric mice that express the BM3.3 TCR only on a fraction of the CD8 T-cell pool.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com