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When applied TTAS with 300 pg of ChIP DNA from mouse tissues, we achieved 86.1% peak overlap between replicates, and with good concordance to reference.
To test anti-VRK1 antibodies in mouse tissues, we examined testis samples from wild-type mice.
To obtain more insight into the prominin-1 SV expression in mouse tissues, we decided to study their expression on mRNA level.
To examine the effects of global p53 gene deletion at specific ages in mouse tissues, we employed the strategy outlined in Fig. 1A.
To detect human neuroblastoma tumors in mouse tissues, we performed immunohistochemical staining using a mouse monoclonal antibody that recognizes human mitochondrial protein (Chemicon, Temecula, CA).
In order to study the relative abundance of the different Gata4 mRNA variants in adult mouse tissues, we performed qPCR on RNA derived from several Gata4-expressing organs (Figure 3).
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For co-immunoprecipitation and western blot analysis with mouse tissue, we used three tissue samples for each group.
For experiments with mouse tissue, we loaded all 12 samples from the four groups on the same gel.
The mutant mouse tissue we examined contained one wild-type copy of APC, and the chicken tissue contained two wild-type copies of APC.
To determine the depth of gold microcarrier penetration following bombardment of mouse tissue, we performed histological assessments of abdominal skin 3d following biolistic gene transfer.
While the focus of our work was mouse tissue, we included supplementary data derived from normal human GIT epithelium where we failed to detect epithelial RUNX3 (Levanon et al, 2011).
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