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Validating this hypothesis, Su and colleagues performed large scale transcription profiling using microarrays on 61 mouse tissues, showing that Dlk1, Meg3/Gtl2, Anti-Peg11, Irm, and Meg9/Mirg are in a genomic region of coordinate gene expression [82].
Smemo et al. [ 102] then established that the sequences have enhancer activity in relevant mouse tissues, showing that expression of Irx3 depends on long-range elements.
Finally, the study includes a comprehensive expression analysis of SAFB1 and SAFB2 in mouse tissues, showing that they have shared but also unique target tissues.
Generation of paralog-specific antibodies allowed extensive expression analysis of SAFB1 and SAFB2 in mouse tissues, showing high expression of both SAFB1 and SAFB2 in the immune system, and in hormonally controlled tissues, with especially high expression of SAFB2 in the male reproductive tract.
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For example, western blot analysis of adult mouse tissues showed Mecp2 expression in the colon [20], and the same authors detected northern blot signal for Mecp2 in the colon but not in the small intestine from humans.
Screening of mouse tissues showed a variety of expression patterns.
Northern blot analysis in adult mouse tissues showed that CA VII is prominently expressed in the mature brain.
Northern analyses of adult mouse tissues showed the presence of two Dazap1 transcripts of 1.75 kb and 2.4 kb, respectively.
RT-PCR analysis on 48 distinct mouse tissues showed that Spatial is highly expressed in the thymus and testis.
In situ hybridization analysis of various mouse tissues showed predominant IPAS expression in the avascular corneal epithelium, correlating with low levels of VEGF gene expression under hypoxic conditions.
Previous reports concerning FGF8 expression in adult mouse tissues showed only a small amount of FGF8 expression in ovaries and testes (Lorenzi et al, 1995).
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