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Extracts of several mouse tissues revealed a complex pattern of interacting proteins, some of which probably resulting from non-specific, hydrophobic binding, while others representing bona fide Leucettine-interacting proteins.
Microarray analysis of an extensive panel of normal adult mouse tissues revealed detectable expression of Sulf1 in nearly all sites examined, with uterus, bladder, mesenteric lymph nodes, prostate, esophagus, lung, mammary gland, and bone showing particularly strong expression (Fig. S3).
(A ) Criteria to find SCN-enriched genes among 83 mouse tissues revealed 230 probesets among which 13 were transcription factors.
RT-PCR expression analysis in mouse tissues revealed a testis-specific transcript of glucose phosphate isomerase, representative of Gpi1_v2 and/or Gpi1-rs1.
In contrast, western blot of a panel of 9 mouse tissues revealed the presence of NAGS, CPS1, and OTC proteins in the liver but only CPS1 and OTC in the intestine (data not shown).
Analysis of a panel of mouse tissues revealed that Nrg4 was highly expressed in adipose tissues, being the highest in BAT, followed by gonadal and subcutaneous WAT (Fig. 8 A ). Analysis of in vivo protein expression by immunohistochemistry revealed the presence of NRG4 in BAT and gonadal WAT (Fig. 8 B ).
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RT-PCR analysis of the mutant mouse tissue revealed that the mutant allele is expressed in the knock-in mice.
Altogether, these data indicate that protein detection of prominin-1 SVs in different mouse tissue reveals the expression of different SVs.
Studies of BRCA1 expression patterns in mouse tissue reveal that BRCA1 is most highly expressed in tissues undergoing rapid proliferation and differentiation, and that expression in vivo is also hormone responsive.
Unexpectedly, our Western blot analysis with fractionated cellular compartments from mouse cerebellar tissues revealed mitochondrial HMGB1 in addition to the nuclear and cytosolic HMGB1 (Fig 2A).
Scanning the mice and the tissues revealed that the uptake of CF750-A33scFv-Fc by the COLO205 tumor graft was significantly less than that by the LS174T tumor graft).
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