Further study revealed that plant MIR168a and MIR156a were detected in various mouse tissues, including liver, small intestine, and lung (Figure 1H and 1I).
To examine molecular events associated with aging and its retardation by caloric restriction (CR), we have employed high-density oligonucleotide microarrays to define transcriptional patterns in mouse tissues, including skeletal muscle, brain, heart, and adipose.
Northern analysis showed that Dlk1 is expressed in many adult mouse tissues, including brain, kidney, testis, and thymus.
Genomic DNA samples from various mouse tissues, including pancreatic islets, were bisulfite-treated and analyzed by methylation-specific PCR (Table 1) followed by sequencing.
Similar results were obtained with mouse MSCs, when the gene/β-actin ratios were compared to the expression of KP enzymes in mouse tissues including the brain, testis, kidney, liver, thymus, spleen and PBMCs (Tables 1, S2).
Indeed, it is widely expressed in human and mouse tissues, including the nervous system, the thymus, various epithelia, and ossification centers, suggesting a widespread functional significance, not restricted to vascular tissues [9], [10], [11].
In our own lab, we have verified the presence of Il7 mRNA by RT-PCR from homogenized mouse tissue including thymus, spleen, lymph nodes and bone marrow (R.I. Mazzucchelli, unpublished observations).
We determined μs′ and γ for 4 different types of mouse tissue, including skin, liver, normal tongue and oral squamous cell carcinoma.
Sample preparations and data analysis for 3′-seq were performed as previously reported [ 22] using total RNA extracted from 8-week-old C57BL/6 male mouse tissue, including testis, liver, brain and skeletal muscle.
In fact, very recent data suggest that telomeres might be sources of persistent DDR even without overt telomere shortening in various mice tissues including the brain (Fumagalli et al., 2012; Hewitt et al., 2012).
On the contrary, the results obtained on thin sections of unirradiated mice tissues including brain, lung, heart, and small intestine have shown that they are almost completely negative for γH2AX (Rübe et al. 2008).
