IHC of thin sections of mouse mammary gland tissues containing human-like DCIS lesions was performed using standard avidin-biotin-peroxidase (ABC) methods.
92.6% infected tissues were identified from the tissues of healthy mice and the tissues containing more than 100 CFU/ml bacteria could be detected by the biosensor.
Recently, it was demonstrated that a subset of sensory axons in adult rat and mouse cutaneous tissues contained an activated form the mammalian target of rapamycin, a serine/threonine protein kinase phosphorylated at serine 2448 (phospho-mTOR, P-mTOR) (Jimenez-Diaz et al., 2008; Geranton et al., 2009; Melemedjian et al., 2011; Obara et al., 2011a, 2011b; Verma et al., 2005).
Brain tissues containing mouse PRh were homogenized in ice with the electronic tissue grinder (IKA® ULTRA-TURRAX® dispersers, Germany) in 10-fold volume of A68 buffer (10 mM Tris HCl pH 7.4, 0.8 M NaCl, 1 Mm EGTA and 10% sucrose) containing protease inhibitor cocktail (Roche).
We evaluated the accuracy of NeuronStitcher using carefully generated "ground truth" reconstructions from a piece of mouse brain tissue containing a labeled pyramidal neuron within the hippocampal CA1 region (Supplement, Section 4.1).
Thus, we co-aggregated and transplanted ESC-derived meiotic oocytes with newborn mouse ovarian tissue containing somatic granulosa cells that are primed to direct follicle formation and oocyte development.
A mouse tissue array containing cores of cortical tissue from wild-type C57BL/6J mice at a variety of prenatal, perinatal, and postnatal timepoints, was immunofluorescently stained for MeCP2 and EGR2 protein and fluorescence was quantified to determine the developmental ontogeny of MeCP2 and EGR2 in mouse cortex.
We also found that the transplanted tissues contained mouse CD31+ cells forming duct-like structures (Fig. 7B), which indicated that transplanted tissue also contained mouse blood vessels.
Our preliminary data shows that mouse liver tissue contains A relatively small amount of microRNAs.
Our preliminary data shows that mouse liver tissue contains relatively small amounts of microRNAs.
