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Using the same method, the MNPs mixed in different homogenates of mouse tissue were collected under magnetic field after proteinase K digestion and carbonated followed by acidification and colorization.
Frozen sections of mouse tissue were fixed in ice-cold methanol, washed in PBS, incubated with 1% H2O2 to quench endogenous peroxidases and blocked in 3% bovine serum albumin (BSA)+20% horse serum in PBS for 30 min. To reduce background signal from endogenous biotin in the pancreas, an avidin/biotin blocking kit SP-2001/Vector SP-2001/Vector Laboratoriesbefore addition of antibodies.
Significant differences in relative areas stained and mean specific intensity for the stains between control and treatment groups in mouse tissue were tabulated.
Significant differences in relative areas stained and mean specific intensity for the stains between control and treatment groups in mouse tissue were calculated.
For the qPCR titration experiments, the entire section of human and mouse tissue were dissected separately, treated with Proteinase K, and then mixed at the ratios shown.
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Mouse tissue was provided to Celera by the Jackson Laboratory of Bar Harbor, Me., a leading mouse colony.
Mouse tissue was fixed by transcardial perfusion of 4% paraformaldehyde (PFA) in PBS.
Saline buffer-treated mouse tissue was used for background fluorescence detection.
RNA from mouse tissue was isolated using TRIZOL (Invitrogen) according to standard protocols.
DCX-immunohistochemistry on mouse tissue was performed as described previously [40].
The distribution of the recombinant viruses in mouse tissue was reported in Fig. 5.
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