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Immunohistochemistry was performed using OCT-embedded, 4um thick acetone fixed mouse tissue sections.
Untreated mouse tissue sections and sections with omitted primary or secondary antibody were used as negative controls.
Paraffin-embedded H69 xenograft mouse tissue sections (4 µm) were dewaxed in xylene and subsequently rehydrated in ethanol.
Immunohistochemistry studies of various human and mouse tissue sections were carried out as described in Methods, with the control IgY in comparison.
The 3F4 mAb was used on the cheetah alone, while SAF84 mAb was used on cheetah and mouse tissue sections as previously described [2], [13], [39].
Mouse tissue sections obtained from several NMRI mice (at the Animal Care Center of the University of Tampere, Finland) were cut into 3 µm-thick sections and dried onto Superfrost Plus™ microscope slides.
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Spectra were collected in transmission mode with the ×15 optics at 4��cm−1 as spectral resolution and 32 scan co-additions for the human tissue section (vs 128 background scans) and 8 sample scans for the mouse tissue section (vs 8 background scans).
Moreover, compared with vehicle-treated mice, tissue sections of liver from mice administered PCB-77 exhibited lipid-laden vacuoles.
To determine the phenotypic characterization of dARKO/Pten+/− mice, tissue sections were sequentially stained with H&E and IHC for characterization.
To evaluate whether apoptotic cell death was altered in IL-1RI KO mice, tissue sections were stained with an Ab specific for activated caspase 3. The amount of activated caspase 3 staining was similar between IL-1RI KO and WT mice (results not shown), indicating that the IL-1RI pathway has no impact on apoptotic cell death during early brain abscess development in this model system.
Superoxide production in mouse aortic tissue sections was detected in-situ [21].
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mouse midbrain sections
mouse brain sections
mouse heart sections
mouse lung sections
mouse liver sections
mouse tissue samples
mouse limb sections
mouse embryo sections
mouse kidney sections
mouse atlas sections
mouse tissue eQTLs
mouse tissue specimens
mouse lens sections
mouse cryostat sections
mouse tumor sections
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