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Although multiple methods are frequently used to detect and quantify infectious MCMV in mouse tissue samples collected during acute viral infection, these are not readily adaptable to high-throughput screening strategies.
The localization of CA XV was studied by immunohistochemistry in mouse tissue samples.
Apoptosis in mouse tissue samples was analyzed by the DeadEnd Fluorometric TUNEL System (Promega, Madison, WI) and by the Colorimetric CaspACE-3 Assay System (Promega).
Total 14C was measured, using a scintillation spectrometer, in feces, urine, and (eventually) in mouse tissue samples that were collected either 48 hr or 15 days post-gavage.
For collecting mouse tissue samples from jet-lagged mice, mice were placed in 24hr LD cycles for two days after jet-lag prior to tissue isolation at indicated ZT times.
We thank M. Talamini, B. Meseck-Selchow, F. Carew-Jones and the staff at our animal facility (ZFE Frankfurt) for technical assistance, and T.P. Velavan for help with sequential extraction of mouse tissue samples.
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Amplification of the human beta-globin gene was used to quantify the amount of human DNA in each mouse tissue sample.
To illustrate the capability of the system to produce a long range B-scan, in vitro imaging of a formalin fixed C57BL/J mouse tissue sample was performed.
To account for this variability, we down-sampled each mouse tissue sample to 25 million reads (random sampling, no replacement) and subsequent datasets were used for DNase I peak and hotspot calling.
For western blotting of mouse tissues, samples were harvested and lysed in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate and 0.1% SDS) using a dounce homogenizer, then briefly sonicated and incubated on ice for 15 min. Pellets were spun down and the supernatant was collected and quantified using a Bradford assay.
Table 3 Standard curves, correlation coefficients, and linear ranges of DTX in mice tissue samples.
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