The use of iTRAQ coupled with α4 and β2 nAChR subunit transgenic mouse tissue provides a significant technical advance over previous proteomic methods used to identify specific nAChR-interacting proteins.
In the current FANTOM5 project, a modified protocol of CAGE for high-throughput single molecule next-generation sequencing with Helicos (hCAGE) has been applied to a wide range of human and mouse tissues providing an unprecedented dataset for promoter usage analysis [ 11].
Proteomic analysis of Ethe1−/− mice tissues provides evidence for a crucial role of the mitochondrial sulfide oxidation pathway in sulfide signaling.
In addition, we found that P. berghei ANKA-GFP iRBC are able to bind to receptors present in mouse placental tissues providing the basis for a pathology trigger of mouse placenta pathology, comparable to the mechanism proposed for human placental malaria.
Mouse tissue was provided to Celera by the Jackson Laboratory of Bar Harbor, Me., a leading mouse colony.
Skeletal muscle data used in the meta-analysis was generated from mouse, rat, and human tissues, providing evidence that the results are consistent across species and platforms.
The authors apply this method to the analysis of amyloid plaques both in mouse tissue and in post-mortem specimens providing proof-of-principle of the reliability of the technique.
We thank Dr. P. A. Overbeek, W. Harrison and D. Liang for providing mouse tissues and for insightful comments concerning this manuscript, Dr. G. Tarone for providing the anti E-cadherin monoclonal antibody, and Drs. G. Gambini and F. Ghiotti for helpful discussions.
Genetically modified mice carrying engrafted human tissues provide useful models to study human cell biology in physiologically relevant contexts.
AG provided assistance with the RT-qPCR experiments, generated the mouse tissue and olfactory tissue sections, ran mesodissection experiments, and provided manuscript review.
