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To further study Wnt4 function during development, we used a Cre/loxP system to conditionally express Wnt4 from the ubiquitously-expressed Rosa26 locus potentially in any mouse tissue in a Cre-dependent manner.
Briefly, human tissue was homogenized in 10 vol and mouse tissue in 5 vol of TBS+ (Tris-buffered saline plus Complete protease inhibitor; Roche Diagnostics, Mannheim, Germany) and spun for 30 min at 120.000×g.
Injection of equal amounts of PERKΔC cells (n = 20, total of two independent experiments) in the s.c. mouse tissue in the region of the abdominal mammary glands did not produce any tumor nodules at the same times, highlighting the importance of an orthotopic microenvironment (data not shown).
Injection of rAAV into scid mice (DNA-PKcs negative) showed a persistence of linear episomal rAAV DNA containing free ends in mouse tissue, in contrast to normal mice, in which all of the free ends had been joined predominantly in a head to tail fashion [78], [79].
This is in accordance with a previous study of mouse tissue in which CDR2 mRNA was widely expressed using Northern blot analysis [ 3].
A similar increase in vasculature was therefore seen in the TSP-2-null mouse tissue in the margin of the disc.
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Variation in circadian cycling of the transcriptome and proteome of mouse tissues in vivo has been demonstrated before, for instance in the liver [7, 26].
The possibility of differential PVL induction in human and mouse tissues in human and mouse response is another potential confounding factor in extrapolating animal data to the human condition.
Because of the discrepancy between patients' PBMCs and KIKI mouse tissues in the relative response to compounds 109 and 136, we compared the effect of HDACi 106, 109 and 136 in splenocytes collected from pooled KIKI or WT mice spleens.
injected antagomirs showed a broad biodistribution and miRNA silencing in many mouse tissues in vivo.
Ltbp3 transcripts were also present in adult mouse tissues in heart, brain, lung and kidney (7, 8).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com