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We used mouse tissue expression database (Su et al., 2004) to identify SCN-enriched transcripts.
To demonstrate the biological validity of tissue meta-profiles, we carried out a principle component analysis of a mouse tissue expression dataset which had been summarized from more than 3000 Affymetrix array datasets available in GENEVESTIGATOR.
Because Northern blot analyses for particular gene loci have previously shown that NATs tend to be poly(A -negative [ 24], we checked whether our A -negative also showed this tendency in normal mouse tissue expression profiling.
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In this study, we compared one corresponding tissue based method and three co-expression based methods for assessing conservation of gene expression, in terms of their pair-wise agreements, using a frequently used human-mouse tissue expression dataset.
Using 16 different tissues from 5-month Hdh Q 111/+ mice as our training set, with instability index as a quantitative phenotype, we analyzed mouse tissue gene expression data (Mouse Gene Expression Atlas GSE11339, C57BL/6J, 10 weeks) to identify a gene expression signature that correlated with tissue repeat instability.
To determine, if the transcriptional profiles associated with primed microglia are preserved in mouse brain tissue, expression sets of App-Ps1, aged, rTg4510 and ME7 prion infected mice (-/+ LPS) were analyzed.
We used the mouse tissue gene expression database of Genomics Institute of the Novartis Research Foundation (mouse Gene Expression Atlas, GSE11339).
For example, our approach allowed a prediction of an instability index in 78 different tissues and conditions in the mouse tissue gene expression data set (Table 1), a far greater number than has ever been previously measured, providing a comprehensive view of tissue instability.
In addition, we used the Novartis human and mouse tissue gene expression atlas data sets to confirm the cloned EST/mRNA results [ 27]. 3) We limited the classification to protein-coding genes which were manually annotated and reviewed by RefSeq or UniProt database curators.
The fact that not all genes can be replicated in the male data may reflect known differences between female and male mouse liver tissue expression profiles [48].
In control mice, CLN6 tissue expression remained consistent over all ages tested.
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