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For marker expression, standard indirect immunofluorescence staining was performed on 6 μm mouse tissue cryosections.
For lipid staining in mouse tissue, cryosections (10- μm thick) were air-dried, rinsed in 60% isopropanol, and stained with 0.3% Oil Red O (Fisher Biotec, Wembley, WA, Australia) for 15 min at room temperature.
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Using immunostaining on tissue cryosections, we characterized the distribution of MECP2 in 60 cell types of 16 mouse neuronal and non-neuronal tissues.
Immunohistology of tissue cryosections indicated tumor-association of these receptors.
These mice express the green fluoresecent protein (GFP) under the pan-endothelial Tie2 promotor, and GFP fluorescence can be visualized in the vasculature of the brain, liver, lung, spleen, kidney, muscle and other tissue cryosections (Figure 1a)[1a].
To further evaluate MCAT expression, we performed immunofluorescence staining on tissue cryosections (Figure 2).
Transverse cryosections from the forelimb region of Sox11 -/- embryos were used to demonstrate the specificity of the SOX11 mouse monoclonal antibody MRQ-58 from Cell Marque (Rocklin, CA, USA) in mouse tissue.
Immunofluorescence assays were performed on cryosections of mouse tissues.
Cryosections of mouse tissues embedded in acetate buffer (AlleMan Pharma GmbH, Rimbach, Germany) were cut at 7 µm thickness, air dried and processed by routine hematoxylin and eosin staining.
Storage of primary tumor tissues, cryosections, and total RNA isolation were previously described 19.
For cryosections of mouse lung tissue, lungs were harvested and embedded in polyfreeze tissue freezing medium (Polysciences).
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