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Examination of mouse tissue and HD striatal cell cultures revealed the accumulation of intracellular cholesterol.
This was observed in mouse tissue and in cultured striatal neurons expressing a fragment of the mutant huntingtin protein.
Our results demonstrated that the scaffolds were well integrated into the mouse tissue and absorbed the exudates after one week.
Likewise, the replicates allowed sensible quality assessment and the best two arrays for each mouse tissue and three arrays for each human tissue were selected for subsequent analyses.
In the case of mouse tissue and 1321N1 cells, total RNA was extracted using the TRIZOL® Reagent (Invitrogen) according to the manufacturer's instructions.
We thank Dr. Alain Caraty for the generous gift of the antisera, Dr. Stephanie Seminara for access to the Kiss1−/− and Kiss1R−/− mouse tissue, and Ms. Jessica Carroll for technical assistance.
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Dr. Guarente, a scientific adviser to Sirtris, said the Wisconsin team only measured the amount of sirtuin present in mouse tissues, and not the more important factor of whether it had been activated.
We also thank L. Mitchell and K. Hwang for expert assistance in germ-cell purification, M. Poy and M. Stoffel for providing mouse tissues, and D. Weir and R. Choksi for assistance with sequencing.
It was postulated that 'excess' antigen, analogous to pneu-mococcal polysaccharide, may persist for extended periods of time in the mouse tissues, and thus induce an unresponsiveness similar to 'immunological paralysis'6.
We detected the expression of mir-122 across five mouse tissues and the result was consistent with that TaqMan microRNA assay.
The accumulation of the cytoskeletal beta- and gamma-actin mRNAs was determined in a variety of mouse tissues and organs.
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