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Any mouse that lost more than 25% of its initial body weight was euthanatized.
Each animal was assigned a social rank from 1 to 3, where 1 is the most dominant animal that won all pairwise social encounters and 3 is the most subordinate mouse that lost all encounters.
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Histological analyses revealed the presence of squamous papillomas of the posterior tongue and/or buccal surface of all mice that lost >20% body weight (Figure 3A, B).
The group of mice that lost between 10 and 25%% of their body weight by day 13 included some that died by day 21 and some that survived.
Analysis of mice that lost either miR-133a-1 miR-133a-1 miR-133a-1 or miR-133a-2 miR-133a-2 miR-133a-2orevealedpmenthat viabothty under normiRNAsysiologicarecondispensable
None of the mice that lost less than 10%% of their body weight by day 13 died during the observation period.
Using mouse models, Ventakachalam and coworkers demonstrated that mice carrying one functional p53 allele developed tumors but they showed however a milder phenotype than mice that lost both alleles [ 20].
The mice that lost 10 25 % of their body weight by day 13 were not used in the experiments because it was not possible to predict the outcome of these mice between days 14 and 21, with some mice dying and some surviving.
However, mice that lose Lhx1 during embryonic development survive, although they struggle to maintain circadian rhythms when kept in complete darkness.
Any mouse that had lost more than 25% of its body weight was euthanized.
Dying mice were defined as those that lost more than 25%% of their body weight by day 13, and surviving mice were defined as those that lost less than 10%% of their body weight by day 13.
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