To test anti-VRK1 antibodies in mouse tissues, we examined testis samples from wild-type mice.
To determine the molecular events during cadmium exposure, we performed RNA-seq on testis samples of treated and untreated mice.
For microarray profiling of the first wave of spermatogenesis, duplicate testis samples were obtained from postnatal mice at ages of 0, 3, 6, 8, 10, 14, 18, 20, 30, 35, and 56 days postpartum [ 8].
We analyzed testis samples from 6 to 38 day old mice with two-day sampling intervals between 10 and 20 dpp to improve the coverage of meiotic samples.
We measured DNA double-strand breaks (DNA DSBs) and oxidative stress parameters including malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and testis weight and sperm count at 12 h, 21 d and 35 d after irradiation in mouse testis.
To evaluate the system, 55 cDNA clones with predominantly unknown function were selected from a mouse testis cDNA-library.
Testis samples were provided by Ren-Min Hospital, Wuhan University.
Nevertheless, eGFP could clearly detected by immunohistochemistry in TNG mouse testis, but not in the testis of non-transgenic mice.
The histology of Mouse testis was compared with human testis obtained from the Armed Forces Institute of Pathology at Water Reed Army Medical Center AFIPP).
The testis-specific poly(A) polymerase is present in the cytoplasm [9], [10] and nucleus [10] of mouse testis cells.
