We measured DNA double-strand breaks (DNA DSBs) and oxidative stress parameters including malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and testis weight and sperm count at 12 h, 21 d and 35 d after irradiation in mouse testis.
To establish the developmental expression patterns of the 24 genes during spermatogenesis, RT-PCR analysis was performed using mouse testis cDNA obtained at different time-periods after birth (8-84 days).
Significantly lower Irm expression was detected after longer exposure times in adult mouse testis, stomach, skin, heart, and muscle.
After lentiviral transduction, SSCs were transplanted into recipient mouse testis.
To evaluate the system, 55 cDNA clones with predominantly unknown function were selected from a mouse testis cDNA-library.
Nevertheless, eGFP could clearly detected by immunohistochemistry in TNG mouse testis, but not in the testis of non-transgenic mice.
The histology of Mouse testis was compared with human testis obtained from the Armed Forces Institute of Pathology at Water Reed Army Medical Center AFIPP).
The testis-specific poly(A) polymerase is present in the cytoplasm [9], [10] and nucleus [10] of mouse testis cells.
Furthermore, eGFP mRNA and protein were detected in the testis of Nanog reporter mice, which is strong evidence that the Nanog gene is transcribed in mouse testis.
We conclude, therefore, that MAP3K1 is not required for mouse testis determination.
