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DNA was isolated from mouse tails using the Qiaprep DNeasy kit (Qiagen) in accordance with the manufacturer's directions.
Genotype analysis was carried out by PCR analysis of DNA extracted from mouse tails using HCV transgene, Mdr2 and Neo primers (primers sequences are specified in the Table 1).
Genomic DNA was extracted from mouse tails using the QuickExtract kit (Epicentre, Madison, WI).
Genomic DNA was extracted from mouse tails using a Wizard Genomic DNA Purification Kit (Promega) and PCR performed as follows.
The founder mice were mated with C57BL/6 mice and both the transmittance of the transgene and the successful expression of human G0S2 protein were examined by western blot analysis of total cell extracts of mouse tails using one of the anti-G0S2 monoclonal antibodies (clone #3-1) we generated (see section 3.4 and Supplementary Fig. S2).
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DNA was prepared from mouse tail using the DNAeasy kit according to the manufacturer's protocols (Qiagen).
The genotype of all the mice were verified prior to experiments by RT-PCR of mouse tail using the RED-Amp Kit (Sigma).
Peripheral blood was collected from the mouse tail using a heparinised micropipette tip.
Genomic DNA was isolated from mouse tail using a kit, and the protocol suggested by the manufacturer (DNeasy Blood & Tissue Kit, Qiagen, USA).
The levels of parasitaemia were measured from blood samples of the mouse tail using Giemsa-stained blood films.
DNA was isolated from ∼2 mm of mouse tail using the Wizard Genomic DNA purification Kit (Promega, Madison, WI) tail tissue protocol.
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