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In the triple transgenic (Tbx4-rtTA/TetO-Cre/mT-mG) mouse system described above, mGFP expression persists within cells once Cre-mediated floxed-mTomato deletion has occurred.
An achromatic lens with anti-reflection coating was inserted immediately in front of the eye to increase system field-of-view and reduce chromatic aberration, a similar design to the mouse system described by Geng et al. (2012) [ 11].
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In later experiments, mice received a single dose of estradiol by inhalation exposure for 1 hr to an aerosol generated from a solution of E2-BSA β-Estradiol 6- O-carboxymethyl)oxime: BSA (75 ng/ml aerosol solution, ∼30 mol steroid per mol BSA, Sigma) using the mouse exposure system described in Hamada et al., 2003.
To determine the extent of OA in the mutant mice, we used the scoring system described by Pritzker et al. [ 31].
Tissue sections (approximately 50 sections per mouse) were graded using the scoring system described by Glasson et al. Because of the specific procedure taken for OA induction, we focused on the histological evaluation of the medial side that exhibited more severe symptoms than the lateral side.
Although the genetic system described in detail here involves mice, there is ample evidence that the same system operates in man.
We used the selection system described above to isolate the Tg.AC transgene from the mouse genome.
The permeability disorders of the vascular system described for ruminants [35] is reflected in the petecheias observed in spleen in BTV infected mice.
The biochemical system described here retains the key characteristics of somatic expansions from non-proliferating tissues in humans and mice.
The mouse and cell systems described here are potential in vivo and in vitro models to assay pesticides, environmental chemicals, and pharmaceuticals as candidate contributors to PD by disrupting DOPAL detoxification.
To definitively address the issue of whether bona fide naïve B cells express TLRs and are responsive to TLR ligands, we utilized the recently described transgenic mouse system where memory B cells are marked based on YFP expression and can be tracked in vivo [19].
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