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The mouse study presented here was initiated to determine the short- and long-term effects of a maternal high-protein diet in pregnancy or lactation on offspring's body weight, fat accumulation and cardiovascular parameters after weaning.
In contrast to our results of the P/I null mouse study presented in this article, Bullard et al., have previously reported a complete loss of neutrophil migration into the peritoneum of P/I null mice during peritonitis induced by i.p. inoculation of Streptococcus pneumonia [ 13].
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There had been over 60 mice used in our previous study and the present study, but no NOD mouse studied presented anti-SSA antibody in serum – which may related to the mice we bought and also to the methods used to detect the antibodies.
This also explains the high standard deviation in the NMA metric for this region (367 %), since it reflects the analysis of three mouse studies presenting with large distorting tumour xenografts while the remaining five mouse studies present a mean relative error of 20.1 % (SD of 25.7 %).
A previous mouse Znt7 knockout study presented a perplexing scenario regarding the role of ZnT7 in dietary zinc absorption: while the Znt7-null mouse has a low overall zinc level, its tissues are not deficient in zinc.
These mice displayed a neurodegenerative phenotype similar to that observed in hA30Pα-syn mice, but with more severe progression: by 12 months of age, 50% of the hA53Tα-syn mice were affected [51], whereas at this age, none of the hA30Pα-syn mice in our study presented features of disease.
Based on the mouse infection studies presented here and the previous studies showing crossreactivity of anti-PDC-E2 autoantibodies to N. aromaticivorans in humans with PBC, we propose the following conclusions.
The major concern is that the mouse studies as presented are somewhat preliminary and difficult to interpret.
However in these two rat studies CPF was administered for longer periods than in the present mouse study.
Concluding, the human melanoma xenograft scid mouse model presented in this study (i) closely reflects the clinical situation and (ii) underlines the complexity of metastasis formation.
A summary of lesions for all mice in the study is presented in Table 3.
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