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In support of this claim, the D2/3 agonist [3H]-N-propylnorapomorphine ([3H]NPA) proved to be more vulnerable to competition from endogenous dopamine than was the antagonist ligand [11C]raclopride, measured ex vivo in mouse striatum, and subsequently in multi-tracer PET studies of analogous design.
Total RNA was extracted from mouse striatum and cortex using Trizol reagent (Invitrogen, Milano, Italy) according to manufacturer's instructions.
It also unambiguously confirms the degree of segregation of MSNs in the mouse striatum and allows the full exploitation of results obtained with BAC-transgenic mice.
We now report that selective pharmacological activation of mGlu3 receptors enhances the production of GDNF in mouse striatum, and that the potent mGlu2/3 receptor agonist, LY379268, is highly protective in the MPTP model of parkinsonism at doses that up-regulate GDNF.
Overall, the distribution of TH-expressing cells observed in the mouse striatum and amygdala with in situ hybridization at prenatal stages was identical to that in neonates.
As a positive control for COX-2, LPS (2.5 μg) was stereotaxically injected into the mouse striatum and RNA was isolated 6 h later.
Similar(44)
For mouse brain samples, all mice striatum and cortex tissues protein extracts were obtained in the following ice-cold extraction buffer: 50mM Tris.HCl pH 7.4, 250mM NaCl, 5mM EDTA.Na2, 1% Triton-100 with 1× Complete Protease Inhibitor, and then homogenized by electric homogenizer.
Mouse striatum, cortex and cell cultures were homogenized at 4°C in a buffer composed of Tris-HCl pH 7.4, 10 mM; NaCl, 150 mM; EDTA, 5 mM; PMSF, 10 mM; Triton X-100, 1%; leupeptin, 1 µg/ml; aprotinin, 1 µg/ml.
Mouse striatum was dissected and homogenized in 300 µl lysis buffer at 4°C containing 137 mM NaCl, 20 mM Tris, 1% Nonidet P-40, 10% glycerol, 1 mM phenylmethylsulfonylflouride, 10 µg/ml aprotinin, 1 µg/ml leupeptin, and 0.5 mM sodium orthovanadate and then centrifuged at 10,000 g for 10 min at 4°C.
Both meta analyses of control human caudate nucleus and wild type mouse striatum data were very comparable and we obtained median Z-summary values of less than 5 for the preservation of modules of defined sizes.
Ranc et al. [45] have presented a method for rapid analysis of dopamine levels in artificial CSF and mouse striatum samples.
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