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We thank David Baltimore for providing lentiviral plasmids, Tularik, Inc. for IKKα cDNA and Marcy Macdonald for mouse striatal precursor cells.
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We have developed sensitive and specific quantitative methods for the determination of MPP+ in mouse striatal tissue by liquid chromatography/tandem mass spectrometry.
Naïve, temperature-sensitive immortalized mouse striatal cells were a generous gift from Dr. Marcy E. MacDonald [27].
Tissue from the striatal precursor region of E16.5 embryos was dissected into RIPA lysis buffer and disrupted by sonication.
For the mouse striatal cultures, the typical dissociation date of E14.5 still contained cholinergic interneurons.
The mouse striatal cells (STHdh) were obtained from Coriell Cell Repositories (Camden, NJ).
Quantitative real-time RT-PCR was used to analyse Foxp2 expression in the striatal precursor region of E16.5 embryos from such crosses.
Therefore, it is assumed that the upregulation of miR-326 might be regulating the Notch signalling for maintaining the striatal precursor pool in vitro.
Time-mated Sprague-Dawley rats containing embryos at gestation day 18 were used for the isolation of striatal precursor cells from the striatum.
Several groups have shown that FGFs regulate embryonic cortical and striatal precursor cell EGF-R acquisition during development (Ciccolini and Svendsen, 1998; Lillien and Raphael, 2000).
Lastly and notably, in mouse striatal slices, there is a similar profile of endogenous STX1 and p-STX1 expression.
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