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Staining of primary HT 29 tumours was detectable in all mice, but varied considerably in both mouse strains; however, HPA-positive cells were clearly present.
In contrast to the Vα11.1/Vβ8.3-tg and Vα11.1/Vβ8.2-tg mouse strains, however, which express both the α-chains and β-chains of the TCR as transgenes, the galactosylated CII-specific Vβ12-tg mouse is only transgenic for the CII-specific β-chain, which may combine with any endogenous α-chain.
Compared to other mouse strains, however, 2-m SAMR1 mice had fewer B1 apical surfaces (543 ± 34 per mm of dorsal SEZ, n = 3, vs. 1,732 ± 53 in C57BL/6 mice, n = 3) and pinwheels (289 ± 30 per mm, n = 4, vs. 952 ± 45 in C57BL/6 mice, n = 3) (for CD1 mice, see also Mirzadeh et al., 2008; Shook et al., 2012), suggesting potential effects of the SAM genetic background in B1-cell population size.
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Using another mice strain, however, Gook et al. (2001) reported follicular development up to the antral stage after xenograft into non-hypogonadic SCID mice without exogenous gonadotrophin stimulation.
Kinetically, the pattern of IFN-γ looks similar in the two mouse strains with however a 3-fold higher production (p<0.01) in H3R−/− mice.
The study by Maroulakou et al. [ 32] did not specify the mouse strains used however Akt1−/− and Akt2−/− mice are typically in a C57BL/6 background while MMTV-neu and MMTV-PyMT transgenic mice are typically in a FVB background.
Similar to TNF-α, mRNA levels of the matrix protein collagen αI, a marker of hepatic fibrogenic potential, was highest in PUFA-fed mice of both strains; however TLR-2 deficiency significantly enhanced expression in mice fed SAFA when compared to diet-matched wild types (Fig. 7).
However, these mouse strains have not yet been compared for more complex cognitive functions, such as long-term/short-term memory and cognitive flexibility.
Differences between mouse strains might be expected; however, differences between the same strain at different centres, namely between SCID mice at CI and AZ, were more surprising.
At time of sacrifice, the primary tumor weight was similar in both mouse strains (Fig. 6b), however there were significantly more and larger lung metastases in the Rag2−/− γc−/− mice than in the Balb/c mice (Fig. 6c, d).
However, both mouse strains had similar level of passively transferred IgG (Figures 2a, 3a).
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