We have also found that some common mouse strains contain a natural mutation in Bfsp2 that will effectively generate a CP49 knockout.
The targeting vector used to create the Lrp6−/− mouse strain contained the β-galactosidase (β-gal) gene.
An in vivo interaction between Myb and p300 was revealed by the identification of the plt6 mouse strain containing a mutation in the KIX domain.
An E14Tg2a.4 ES cell line (derived from the 129/OlaHsd mouse strain) containing a gene-trap cassette inserted into the Prkd2 locus (RRJ380, Bay Genomics) was injected into C57Bl/6J blastocysts, which were subsequently implanted into recipient female mice.
We applied a proteomic approach to a mouse strain containing a null mutation of the Ugt1 gene with the aim of shedding light on the mechanisms and molecular effectors involved in bilirubin-associated neurotoxicity in vivo.
To distinguish the functions of secreted BMP2 from nBMP2, we generated a mouse strain containing a targeted mutation in which the conventional growth factor BMP2 is secreted and signals normally, but nBMP2 is excluded from the nucleus by means of a three-amino-acid substitution in the nuclear localization signal [ 2].
Standard mating techniques [36], [38] were then used to produce, initially, mouse strains that contained a "tri-lox" Ate1 configuration, in that they also contained the floxed positive-selection PPGK-hph cassette (Fig. 2D).
These mouse strains also contained the CaggCreER gene, expressed from the ubiquitously active chimeric Cagg promoter (Fig. 2E) [25].
During the week prior to TM treatment, Ate1flox/− CaggCreER and control (Ate1flox/+ CaggCreER) mice (at that point, both strains contained Ate1) consumed 0.63 and 0.62 kcal of standard chow per gram of body weight per day, respectively (Fig. 10C).
The transgenic mouse platform employed includes several mouse strains, each containing DNA encoding parts of the human variable region antibody repertoire and varying human constant regions.
