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The lack of adverse effects of both of these defensins on the proliferation of isolated mouse splenocytes indicates that these molecules are selective.
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E.g., -α-pinene, -linalool and -limonene were able to decrease interleukin-2 (IL-2) secretion and to increase the IL-10/IL-2 ratin in mouse primary splenocytes, which indicates their property to repress Th1 immune activation and suggest a potential inclination towards Th2 [ 19].
Our results indicate a correlation between IFN-β1 and TRAIL expression in mouse splenocytes upon LPS treatment in the presence or absence of Smaducin-6 (Fig 7C– F).
Mouse splenocytes obtained from naïve C57BL/6 mouse spleens were made in a single cell suspension through mechanical methods and strained through a 35 µm mesh.
The T lymphocytes were mouse CD4 T cells from whole mouse splenocytes.
Mungin also exerts inhibitory activity against α- and β-glucosidases, suppressing [3H] thymidine in corporation by mouse splenocytes [51].
NOD mouse splenocytes were also transferred alone as controls.
Cell fusion was performed using mouse splenocytes and NSO mouse myeloma cells using standard techniques [67].
Chromosome instability was measured in metaphase spreads prepared from wildtype (mTERC+/+), mTERC+/− and mTERC−/− mouse splenocytes.
In study 3, mouse splenocytes were exposed to the non-cytosolic fraction.
Mouse splenocytes were isolated from the spleen after CLP.
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