Sentence examples for mouse spleen using from inspiring English sources

Naïve B cells were purified from mouse spleen using CD43 Dynabeads (Invitrogen) according to the manufacturer's instructions.

5' and 3' rapid amplification of cDNA ends (RACE) was performed using Marathon-Ready cDNA from mouse spleen using the Advantage cDNA PCR kit (both from Clontech) as described previously [ 30].

Mononuclear cells were isolated from allogenic mouse spleens using gradient separation and plastic-adherence depletion.

Total RNA was isolated from mouse spleens using the RNeasy kit (Qiagen) as recommended by the manufacturer.

Untouched CD4+ T or CD8+ T cells were purified from mouse spleens using the Dynal Mouse CD4 or CD8 Negative Isolation Kit (Invitrogen).

For T-cell IFN- γ release assays, irradiated DCs (1.6 × 10/200  μl per well) purified from nude mouse spleens using CD11c MACS beads (Miltenyi Biotec) were cocultured in 96-well plates with splenic T cells (1.6 × 10/200  μl per well) purified from BALB/c mice using CD90.2 MACS beads (Miltenyi Biotec), and stimulated with an anti-CD3 antibody (1  μg per ml) (BD Biosciences Pharmingen).

For microarray analysis, RNA was prepared from mouse liver and spleen using Trizol Reagent (Invitrogen) as described previously [76].

Moreover, we report that stimulation of mouse spleen cells using either mitogenic or sub-mitogenic levels of anti-CD3/CD28 resulted in a dramatic increase in the level of HuA.

B cells as responder cells were obtained from mouse spleen by using B cell isolation kit (Miltenyi).

Full-length mouse BAFF-R cDNA (GenBank accession number: NM_028075) was amplified from mouse spleen cDNA using primers mBAFF-R4 (5'-ATT AGA TCT GAA ATG GGC GCC AGG AGA CTC C-3') and mBAFF-R5r (5'-GAT GAA TTC CTA TTG CTC TGG GCC AGC TG-3').

CD4+ T cells were purified from suspensions of adult WT mouse spleen cells using the CD4+ T Cell Isolation Kit II (130-095-248; Miltenyi BergischBerGladbachadbach, Germany) and adjusted to 1 × 10 cells/ml in RPMI-based complete medium.