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A 20% homogenate of immune-stimulated mouse spleen tissue was prepared in 10 mMTris-HCl pH 7.5 supplemented with 1 mM EDTA, 100 mM NaCl and 5% sucrose.
Prior to the in vivo study, the stability of quinacrine in mouse spleen tissue was determined.
Twenty-two (96%) of 23 house mouse spleen tissue samples showed evidence of LCMV infection by virus isolation and IFA with specific LCMV antibodies.
Using 3 different doses of quinacrine (4 mg/kg/d, 80 mg/kg/d, 160 mg/kg/d), mouse spleen tissue was soaked in 100% methanol for 7 days.
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No statistical difference in mean bacterial load was detected between A1a infected mice and A1b infected mice in either blood or spleen tissue.
Tissue samples for western analysis were not available from RIII mice infected with the MM blood donor spleen tissue and samples from RIII mice infected with the MV blood recipient brain tissue were negative by the concentration method.
Moreover, inoculation of vCJD-infected human spleen tissue killed tg650 mice within 570±63 days (5/5), which indicates an infectivity level approaching that in tg650 mouse spleen.
In contrast, infectivity levels 100- to 1000-fold lower than in the brain were reported for vCJD-infected human spleen tissue through titration in RIII mice [23].
(a) Lung tissue, (b) spleen tissue, and (c) liver tissue.
At 3 4 months of age (a time point when lpr mice develop lupus), MRL-lpr, B6-lpr, NZB/W and their control mice were euthanized and spleen tissues were collected to isolate splenocytes.
We have also identified several RNase L-interacting proteins from natural mammalian tissue like mouse spleen [ 30].
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