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One study on mammals showed a significant decline in mouse spermatogonial stem cells following the administration of Ag NPs [17].
To study the ability of human spermatogonial stem cells (hSSCs) to proliferate in vitro under mouse spermatogonial stem cell (mSSC) culture conditions.
The AgNPs as contaminants effect a decline in proliferation of mouse spermatogonial stem cell (C18-4) (Braydich-Stolle et al., 2010) and cause mitochondrial dysfunction and induction of ROS which in turn set off DNA damage and chromosomal aberrations resulting in cell cycle arrest (Asharani, Lian Wu, Gong, & Valiyaveettil, 2008).
The Ras-cyclin D2 pathway is involved in mouse spermatogonial stem cell development in vitro [42].
In mammals, it appears to be important for self-renewal of human embryonic stem cells [14] and mouse spermatogonial stem cells (SSCs) [15].
To investigate whether tilapia spermatogonia could be cryopreserved and, after thawing, generate normal spermatogenesis following transplantation to a recipient, germ cells suspensions from adult fish were subjected to the standard procedure used for freezing mouse spermatogonial stem cells [18].
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In the Stra8-EGFP transgenic mice, spermatogonial stem cells can be labeled by EGFP as characterized by transplantation assay [ 14].
GDNF and FGF2 also support long-term proliferation of mouse and rat spermatogonial stem cells on fibroblast feeder layers in serum-free media.
In the adult mouse testis, spermatogenesis originates from spermatogonial stem cells (Asingle, As), that can self-renew or differentiate into committed paired (Ap) and aligned (Aal) spermatogonia.
To evaluate colonizing ability of human spermatogonial stem cells in mouse testes.
CDH1 is encoded by Cdh1 for e-cadherin, and it has been regarded as a maker for spermatogonial stem cells in mice.
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