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Author's response: We validated all motifs used for our similarity comparisons with the references databases JASPAR and UniProbe for mouse species using TOMTOM program.
One way to solve this issue would be to study substitution patterns in the mouse lineage by comparing two closely related mouse species, using rat as an outgroup.
We validated all motifs used for similarity comparisons with two reference databases: JASPAR [ 30] and UniProbe [ 33] for mouse species using TOMTOM [ 36] program with P-value cutoff ≤ 0.01.
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The mice species used in the study may carry some similarities to our own biology because they're mammals.
To discriminate between endogenous and exogenous expression of the stem cell genes across species, different primers were generated for mouse and the non-mammalian species, using non-overlapping sequences.
tAI of human and mouse orthologues was calculated for each species using its own genomic tRNA pool (Chan and Lowe 2009).
We also have investigated the anti-inflammatory effects of the three Glycine species using lipopolysaccharide (LPS -stimulated mouse macrophage (RAW264.7) ex vivo.
These results are unlikely to indicate formaldehyde leukemogenicity, particularly when weighed against the relatively high background rates of hematopoietic malignancies in the mouse and rat species used in these studies and the overwhelmingly negative results from nine other carcinogenicity studies in rats and mice.
However, it may be possible that the age (young vs. adult animals) and the species used (mouse vs. rat) may contribute to these differences.
It may well be possible that the varying results of these studies could depend on the different experimental models (in vitro vs. in vivo studies) or to the different animal species used (mouse vs. rat).
The mouse is the major species used in AD and FTD research, but significant additional insight has been provided from species such as the roundworm Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and two types of fish, the sea lamprey and the zebrafish (Götz et al., 2007).
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