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The mouse skull was left intact and a saline-filled vasoline well covered by a glass coverslip was made as an imaging window.
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The approach of measuring distances between defined points on three-dimensional CT image reconstructions of mouse skulls was successfully employed in studying mouse models for Down syndrome (Arron et al, 2006; Richtsmeier et al, 2002).
Before sacrificing the mouse, the skull was removed to expose both sides of the brain tissues and a last in vivo image was obtained.
The frontoparietal skull was exposed and the mouse head was immobilized in a custom stereotactic holder.
For mice outfitted with a stainless steel cannula connected to a micro-osmotic pump, the skull was removed after cannula removal.
The skull was exposed and cleaned.
The skull was without pathological finding.
The skull was opened for CSF collection.
The skull was then cleaned with Betadine.
The skull was shortened and dome-shaped.
Even though a technique has been published which creates an insult adjacent to a cranial window on a mouse skull, such approach is unlikely to permit serial injections [ 16].
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