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p-DDAP caused an increase in epidermal thickness and decreased matrix metalloprotease and hyaluronidase activities in mouse skin tissues to the same extent that RA did.
IFN-γ concentrations in un-infected mouse skin tissues were undetectable (Figure 5B).
As shown in Figure 2A, in vehicle (DMSO) treated mice, whether on Basal diet or Protandim diet, few macrophages were observed in the mouse skin tissues.
We further visualized the expression patterns of RASSF9 protein in mouse skin tissues by double immunofluorescence staining of frozen sections using RASSF9- and K1-specific antibodies.
To monitor the possibility of mycoplasma infection in the mouse skin tissues, the levels of mycoplasma pathogens were detected in a variety of tissue lysate (e.g., whole cell lysate, nuclear extract) using a MycoAlert Mycoplasma Detection Kit purchased from Lonza (Rockland, ME), and the results were negative.
The cDNAs for mCLSPs were PCR-amplified from mRNA of mouse skin tissues.
Similar(51)
Indeed, quantitative analysis of p21Cip1 (CDKN1A) mRNA expression in mouse skin tissue by QRT-PCR revealed a general decrease of p21Cip1 mRNA in the skin of RASSF9−/− mice (Figure S9).
A similar phenomenon has been previously reported in normal mouse skin tissue [ 33, 36] and was attributable to a Raman shift.
In a comparative study, primary cell cultures of neonatal (1 2 day old) mice skin tissue, supplemented with 10% fetal calf serum, were seeded onto locust chitosan based scaffolds (LCSBS) and shrimp chitosan based scaffold (SCSBS).
Collectively, the data in Fig. 7 indicate that P2X7 receptor expression levels in mouse skin cancer tissues are four-five folowerwer than in mouse normal skin tissues.
To visualize cross-sections of mouse skin, whole skin tissue was embedded in O.C.T. compound (Tissue-Tek), and 10 µm skin sections were cut on a Leica Cryostat.
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