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After incubation for 1 hour in mouse serum, the radiolabeled peptide was ∼95% intact and slowly degraded over time to ∼30% after incubation in serum for 24 hours.
For detecting anti-BCR/ABL antibodies in mouse serum, the tests were performed.
For analysis of human and mouse serum the Caenorhabditis elegans miRNA cel-miR-39 is typically used (5′-UCACCGGGUGUAAAUCAGCUUG).
In the case of the mouse serum, the addition of C data not only enhanced our ability to identify specific compounds but it also prevented mistakes that could have been made with H data alone.
After a blocking stage (10%% normal mouse serum), the tissues were incubated with biotin-labelled 4G8 anti-Aβ antibody (Covance), revealed with DyLight 488-labelled streptavidin (KPL, Eurobio, France).
We can see that the system maximally responds to changes in concentration of Epo in the interval 10-3-10-1, which inormals the norangerange of Epo concentration in mouse serum (the behaviour is similar in case of pEpJ [see Additional file 1]).
Similar(53)
The dilution of the mouse serum, as the detector antibody, was optimized following the same strategy.
TGF-β3 neutralizer added to the medium containing mouse serum inhibited the effect.
Normal mouse serum as the primary antibody was used for the negative control.
After suspension in phosphate-buffered saline (PBS) + 0.2% normal mouse serum (NMS), the bacteria were counted and diluted to the desired concentrations for inoculation.
The integral analysis of the inflammation elicited by Bbil-TX in the mouse serum performed in the present study allowed a parallel evaluation of the increase in microvascular permeability and the production of various inflammatory mediators.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com