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The larger bands were also co-immunoprecipitated with FK2, but not with mouse serum, indicating that the larger bands were probably Ub-LlPTPCs.
Furthermore, SWAPs treated with anti-SjTGR antibodies expressed a lower TR enzymatic activity than untreated SWAPs, and the enzyme activity was not affected after incubating with normal mouse serum, indicating that anti-SjTGR antibodies influenced the thioredoxin reductase activity of SjTGR to catalyze DNTB into NTB, which can be measured at 412 nm.
We selected candidate miRNAs that satisfied three criteria: (1) the level in DCKO mouse serum was >1.5-fold higher than that in controls; (2) the global normalisation value was >100 in DCKO mouse serum, indicating easily detectable levels of miRNAs; and (3) the identified miRNAs were coincidently upregulated in both sera and DGC tissues.
In contrast to previous observations with irradiated cells, the response to doses above the HRS-range was also affected by the mouse serum, indicating a higher concentration of TGF-β3 in the mouse serum than secreted by LDR primed cells (Edin et al. 2014).
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However, the failure of ELISA measurements of TGF-β3 in mouse serum indicate a concentration below the sensitivity of the assay (~0.1 ng/ml (Edin et al. 2014)).
Both homozygous and heterozygous hema6 mice exhibit elevated total bilirubin concentration in the serum, indicating increased hemolysis.
qRT-PCR for miR-1 and miR-206 in serum indicated that these miRNAs are 20/40-fold 20/40-foldn mdx with renrichedo WT mine (Fig 3D).
Both active bDAb preparations retained their original antigen-binding activity after incubation at 37 °C in mouse serum for up to 7 days, indicating excellent stability of the constructs.
Similarly, recombinant sialidase was not immunoreactive to mouse serum obtained from UV-killed P. acnes-immunized mice (Figure S2), indicating that the undetected immunogenicity was not due to denaturation of sialidase during the heat treatment.
Similarly, SEP54 antibody detected stronger signal in ob/ob mouse serum samples than that in WT mouse sample.
Experimental results indicate that the biosensor is able to detect recombinant H5 HA at 1.4 μM in 10% mouse serum, with high specificity for H5 as compared to H1 (H1N1 A/South Carolina/1/18).
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