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Optimized gene delivery formulations transiently expressed secreted alkaline phosphatase in mouse serum for 12 days.
The di-diabody retained good antigen-binding activity after incubation at 37 °C in mouse serum for 72 h, demonstrating good product stability.
Both active bDAb preparations retained their original antigen-binding activity after incubation at 37 °C in mouse serum for up to 7 days, indicating excellent stability of the constructs.
p97-[14C]ADR conjugate was stable in mouse serum for at least 1 h, with some degradation observed after 18 h (Figure 6a).
Aliquots containing up to 400 µCi of 64Cu-DOTA-knottin 3-4A were incubated in 50% mouse serum for up to 24 h.
Sporozoites were collected from slurry using a renografin gradient (Bracco Diagnostics), and suspended in either M199 media containing 3% naïve mouse serum for injection into BALB/c mice or in lysis solution (RNAqueous Micro kit, Ambion) for RNA extraction.
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Outer TE cells were labeled with IgGs by incubation with 10% rabbit anti-mouse serum for 60 minutes.
Endogenous peroxidase activity was quenched (PBS +2% hydrogen peroxide), and the specimens were sequentially blocked [1% bovine serum albumin/PBS, room temperature 1 hr, followed by mouse serum (Santa Cruz Biotechnology) for 30 min, room temperature].
A GP33-negative LCMV-co-infection (instead of recombinant IFN-I or LCMV-infected mouse serum) might, for example, be useful to test.
Salmonella cells were grown in Luria-Bertani (LB) broth and then opsonized with 1% mouse serum (Innovative Research) for 20 min prior to infection.
The infected cells were incubated with anti-S mouse serum (1 500) for 1 h, and then incubated with FITC-labelled goat anti-mouse IgG (H + L; Zhongahan Co., Beijing, China) for 30 min.
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