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The mouse was sequentially perfused with 10 ml mouse serum, 30 ml mouse serum containing 5% full-strength HP-γ-CD-solubilized curcuminoids, and then washed with 30 ml of PBS.
The remainder of the library was added to 50 μg mL 1 8 week old NOD18 mouse serum containing 1 mM porcine insulin (Sigma-Aldrich) and incubated overnight at 4 °C.
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Invasion assays were performed in either FBS or mouse serum-containing medium (derived from either wild-type, MMP-2-/ or MMP9-/ mice).
The demonstration that NPM could bind to CL prompted us to study the effect of rhNPM on the CL-binding activity of purified 4B7, an aCL mAb derived from a WB mouse whose serum contained both aCL and anti-NPM antibodies [ 10].
Cells were cultured at a density of 1.5 × 10 cells/ml in mouse osmolarity RPMI 1640 medium with 10% (vol/vol) fetal bovine serum containing mouse Flt3L (100 ng/ml, Peprotech) at 37°C in 10% CO2 (Naik et al, 2005).
6– 8 The arthritis is transferrable to naïve mice through injection of serum containing anti-G6PI antibodies, 9 with the resulting inflammatory arthritis in recipient mice resembling the effector phase.
DOI: http://dx.doi.org/10.7554/eLife.06054.008 To determine if protection could be transferred to naïve mice, 250 μl of serum (containing 750 μg of immunoglobulin administered intraperitoneally) or 3 × 10 T cells (administered intravenously) isolated from the blood or spleen and lymph nodes of immunized mice, respectively, were administered to C57BL/6 mice.
Before incubation, the slides were washed for 30 min in 5% normal serum (containing goat, mouse and rat serum, each at 5%; [code numbers X0907, X0910 and X0912; DakoCytomation, Denmark]).
Passive transfer of serum containing arthritogenic autoantibody from K/BxN mice into normal mice also induces arthritis [ 23, 24].
Serum containing arthritogenic autoantibodies from K/BxN mice was used to induce arthritis in mice genetically lacking NMU.
Mouse ESCs were cultured in feeder-free conditions and serum containing media with leukemia inhibitory factor.
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