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There was a 5-fold increase of the fluorescence reading in the AvrA- infected mouse serum compared to that in the PhoPc mouse serum.
Interestingly, the relative luciferase activity values were actually higher for young mouse serum, compared to old (Fig. 3B).
These data clearly demonstrated that while bioactive TGF-β1 was higher in old mouse serum, compared to young, the levels in either sera were minute (> 4 ng mL−1) and unlikely to be inhibitory in the 5 10% range examined (Figs S3C,D and 1).
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Figure 4A shows that lung virus titers were significantly lower in mice that had been treated with SHe-KLH immune serum compared to mice that had received PBS or KLH immune serum.
When the vascular leakage of infected mice was measured by hematocrit 24 hr post-infection, we observed that i.p. challenge with either DENV1 (a heterologous challenge) or DENV2 (a homologous challenge) enhanced vascular leakage in mice that had been adoptively transferred DENV2 post-immune serum, compared to mice adoptively transferred naïve serum.
TH mice expressed substantially increased amounts of leptin in serum compared to B6 mice, which was consistent with the diabetic phenotype of TH mice (Fig. 3A).
Co-administration of nilotinib and doxorubicin to mice decreased the expression of IL-1β RNA in the liver and suppressed the level of IL-6 protein in the serum compared with mice that were injected with doxorubicin alone.
We further demonstrated that co-administration of nilotinib and doxorubicin to wild-type mice was effective in decreasing the expression of IL-1β RNA in the liver and in suppressing the level of IL-6 protein in the serum compared with mice that were injected with doxorubicin alone.
Our study showed that tacrolimus inhibits bone erosion in a serum-induced arthritis mouse model, compared to serum-induced arthritis mice not treated with tacrolimus.
Paraoxonase (PON) 1 arylesterase activity, was higher in Spalax compared to both human and mouse serum levels.
1% mouse serum was added as a protein source.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com