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The genomic ADAR2 human and mouse sequences were analyzed using the UCSC genome browser [22].
In MULTIPIPMAKER, the reference mouse sequences were compared with the corresponding human, chimp, rat and dog genomic sequences in order to identify regions of high conservation across species.
Non-conserved regions were masked, then mouse sequences were collected.
Human and mouse sequences were used as initial query.
Similar rat and mouse sequences were predicted to bind miRNAs when mismatches were allowed [ 32].
All mouse sequences were recently published by Zaballos et al. [ 30].
Similar(42)
The endogenous human and mouse sequences are identical for this peptide.
Whole-genome comparison of human and mouse has indicated that about 5% of human and mouse sequences are under evolutionary constraint [ 41], of which 3.5% are noncoding sequences.
A phylogenetic tree constructed from human and mouse PRAME gene K S values revealed that mouse sequences are monophyletic, as are human sequences.
A plasmid to express RNA without homology to human or mouse sequences was used as a control in silencing experiments [ 31].
For genes for which no ovine sequence was available, a comparative gene alignment of bovine, human, rat and mouse sequences was made and primers were then designed on the most conserved regions between the species.
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