Engelbreth-Holm-Swarm mouse sarcoma basement membrane matrix (3D BM-matrix) was purchased from BD Biosciences.
Matrigel is a commercial product extracted from a mouse sarcoma rich in extracellular matrix proteins.
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm mouse sarcoma, a tumor rich in extracellular matrix proteins.
BD Matrigel, a basement membrane matrix marketed by BD Biosciences is a gelatinous protein mixture secreted by Engelbroth-Hom-Swarm (EHS) mouse sarcoma cells and resembles the complex extracellular matrix environment.
Matrigel, a reconstituted extracellular matrix derived from a mouse sarcoma line, is increasingly being utilized for mammary repopulating assays, although original studies were carried out in its absence.
Matrigel, which is basement membrane matrix derived from Engelbreth-Holm-Swarm mouse sarcoma, and is composed mostly of laminin, collagen type IV, heparan sulphate proteoglycans, entactin and nidogen, was administered to produce local matrix deposition.
For the invasion assay, the inserts were previously coated with extracellular matrix gel from Engelbreth-Holm-Swarm mouse sarcoma (BD Biosciences, Bedford, MA).
For invasion assay, the membrane undersurface was coated with 30 μl ECM (Extracellular matrix) gel from Engelbreth-Holm-Swarm mouse sarcoma (BD Biosciences, Bedford, MA, USA) mixed with RPMI-1640 serum free medium in 1 5 dilution for 4 h at 37 °C.
Endothelial tube formation was assessed using Matrigel™, a solubilized extracellular basement membrane matrix extracted from the Engelbreth-Holm-Swarm mouse sarcoma, as described previously [ 53].
Currently, the most widely used growth substrates for hPSC culture are mouse embryonic fibroblast (MEF) feeder layers or Matrigel™ (an undefined extracellular matrix (ECM) protein mixture harvested from Engelbreth Holm Swarm mouse sarcoma cells).
