TRC clones TRCN0000033260 and TRCN0000033262 were used to silence Kras in mouse sarcoma lines.
Cells from a LPB cell line, a methylcholanthrene-induced C57Bl/6 mouse sarcoma cell line [27], were cultured using standard procedures in MEM (Gibco BRL, Cer-gy-Pontoise, France) supplemented with 100 U.ml−1 penicillin, 100 mg.ml−1 streptomycin (Sarbach, France) and 8% foetal calf serum (Gibco).
The mouse sarcoma cell line S-180 and macrophage cell line RAW264.7 were cultured in Eagle's Minimum Essential Medium and Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated fetal bovine serum, respectively.
The LPB cell line, a methylcholanthrene-induced C57Bl/6 mouse sarcoma cell line (Belehradek et al, 1972), and the B16 F0 melanoma cells (ATCC CRL 6322) were cultured using classical procedures and minimum essential medium culture medium (Gibco BRL, Cergy-Pontoise, France) supplemented with 100 U ml−1 penicillin, 100 mg ml−1 streptomycin (Sarbach, France) and 8% fetal calf serum (Gibco).
The S-180 mouse sarcoma cell line was obtained from the China Cell Culture Centre, grown and maintained in RPMI-1640 medium (Gibco) supplemented with 10% FBS.
Further work by other researchers in the 1980s supported this theory with studies of metastatic subclones from a mouse sarcoma cell line [ 26].
Comparable hTNFα neutralising activity against a TNFα-sensitive mouse sarcoma cell-line (WEHI-164) has also been demonstrated.
Mouse sarcoma cell lines were established from 2 Kras;p16p19 null mouse RMS tumors (T14-R, SMP-01) and one Kras;p16p19 null mouse NMS tumor (Sca1-01).
For the Kd studies using flow cytometry, mouse sarcoma cell lines engineered to express full-length human SAIL were incubated with increasing concentrations of antibodies.
Mouse sarcoma S180 cell line was harvested and the cells were resuspended in NS at 1.0 × 107 cells/mL to 1.3 × 107 cells/mL.
